In vitro maintenance of different isolates of Leishmania parasites for various research activities.

• Bacterial culture of urine and their drug sensitivity.
• Culture of bone marrow or splenic aspirate and biopsy of skin lesion of KA & PKDL patients, respectively for the primary isolation of promastigotes.
  
  

• Showed diurnal periodicity of Leishmania amastigotes in peripheral blood of Kala-azar patients.
• Developed sensitive media for primary isolation of L. donovani from bone marrow aspirate and developed completely autoclavable media for in vitro maintenance.
• Developed a simple technique to remove contamination from Leishmania culture without using antibiotics / anti fungal agents.
• Technique for cytokine production by using Leishmania parasites.
• Preservation of L. donovani promastigotes by a simple method up to 7 months in blood agar slants.
• A pilot study on the antileishmanial activity of metabolites of bacteria isolated from sand fly gut.
  

• In vitro role of Leishmania isolates of responsive and unresponsive patients in INF-γ and IL-4 production by similar sets of T – cells.
• In vitro, Drug (SAG) sensitivity test on intracellular amastigotes of L. donovani and its comparison with clinical response of the similar isolates.
• Crucial role of plants-extract in the development of Leishmania donovani promastigotes.
• Antileishmanial metabolites from the bacteria of sand fly (Phlebotomus argentipes) gut.
• Cloning of Leishmania on solid medium.
• Antileishmanial compounds from Pro and Eukaryotic Micro algae – A search.
• Use of clot or sedimented cells of human discard blood as an easily available & economical alternative to Foetal Bovine Serum or fresh rabbit blood in completely autoclavable medium for culture of Leishmania donovani promastigotes.