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HOD (Microbiology)
Email: gupta1ak@yahoo.com
Ph.: +91-612-2638062
Fax: +91-612-2634379

Mr. A. K. Gupta
 
A Microscopic view of amastigotes of L.donovani in Bone-marrow aspirates
The division is working in different aspects of Microbiology / Parasitology, Immunology & Molecular Biology of Leishmania donovani.
 
The Scientific Team
Name Qualification          Designation
M.Sc. Scientist E
Dr. Vahab Ali
M.Sc., M. Phil., Ph.D. Scientist E
M.Sc., Ph.D. Scientist D
Dr. Shubhankar Kumar Singh    
M.Sc., Ph.D. Scientist D
The Students Team
Name Qualification          Designation
Mr. Ajit Kumar Thakur M.Sc. (Biotechnology) Ph.D. Scholar
Mr. Pushkar Shivam M.Sc. (Biotechnology) Ph.D. Scholar
Ms. Fauzia Jamal M.Sc. (Biotechnology) Ph.D. Scholar
Ms. Pushpanjali M.Sc. (Bimedical Genetics) Ph.D. Scholar
Ms. Sarita Kumari M.Sc. (Biotechnology) Ph.D. Scholar
Mr. Gufran Ahmad M.Sc. (Biotechnology) Ph.D. Scholar
Mr. Manish Kumar M.Sc. (Biotechnology) Ph. D. Scholar
Facilities & Services:

In vitro maintenance of different isolates of Leishmania parasites for various research activities.

  • Bacterial culture of urine and their drug sensitivity.
  • Culture of bone marrow or splenic aspirate and biopsy of skin lesion of KA & PKDL patients, respectively for the primary isolation of promastigotes.

Major achievements
  • Showed diurnal periodicity of Leishmania amastigotes in peripheral blood of Kala-azar patients.
  • Developed sensitive media for primary isolation of L. donovani from bone marrow aspirate and developed completely autoclavable media for in vitro maintenance.
  • Developed a simple technique to remove contamination from Leishmania culture without using antibiotics / anti fungal agents.
  • Technique for cytokine production by using Leishmania parasites.
  • Preservation of L. donovani promastigotes by a simple method up to 7 months in blood agar slants.
  • A pilot study on the antileishmanial activity of metabolites of bacteria isolated from sand fly gut.
 
Ongoing Activities : 
  • In vitro role of Leishmania isolates of responsive and unresponsive patients in INF-γ and IL-4 production by similar sets of T – cells.
  • In vitro, Drug (SAG) sensitivity test on intracellular amastigotes of L. donovani and its comparison with clinical response of the similar isolates.
  • Crucial role of plants-extract in the development of Leishmania donovani promastigotes.
  • Antileishmanial metabolites from the bacteria of sand fly (Phlebotomus argentipes) gut.
  • Cloning of Leishmania on solid medium.
  • Antileishmanial compounds from Pro and Eukaryotic Micro algae – A search.
  • Use of clot or sedimented cells of human discard blood as an easily available & economical alternative to Foetal Bovine Serum or fresh rabbit blood in completely autoclavable medium for culture of Leishmania donovani promastigotes.